The clinical benefits of the CryoTek process have been demonstrated in more than 400 peer-reviewed publications. By devitalizing the resident living cells, CryoTek helps preserve the key components of the extracellular matrix:^6-8

• HC-HA/PTX3
• Collagen (types I, III, IV, V and VI)
• Fibronectin
• Laminin
• Hyaluronic Acid
• Proteoglycans
• Growth Factors

The goal of preserving the structural integrity of the human tissue is to closely maintain the properties of fresh birth tissue.⁹

CryoTek processed UC/AM Tissue

• Tseng SCG. HC‑HA/PTX3 purified from amniotic membrane as novel regenerative matrix: insight into relationship between inflammation and regeneration. Invest Ophthalmol Vis Sci. 2016;57(5):ORSFh1–ORSFh8.
• He H, Li W, Tseng DY, Zhang S, Chen SY, Day AJ, Tseng SCG. Biochemical characterization and function of complexes formed by hyaluronan and the heavy chains of inter‑α‑inhibitor purified from extracts of human amniotic membrane. J Biol Chem. 2009;284(30):20136‑20146
• He H, Zhang S, Tighe S, Son J, Tseng SCG. Immobilized heavy chain–hyaluronic acid polarizes lipopolysaccharide‑activated macrophages toward M2 phenotype. J Biol Chem. 2013;288(35):25792‑25803.
• Zhang S, He H, Day AJ, Tseng SCG. Constitutive expression of inter‑α‑inhibitor heavy chain 1 is required for the formation of the heavy chain–hyaluronan complex in human amniotic membrane. J Biol Chem. 2012;287(15):12433‑12444.
• Zhang S, Zhu YT, Chen SY, He H, Tseng SCG. Constitutive expression of inter‑α‑inhibitor heavy chain 1 is required for the formation of the heavy chain–hyaluronan complex in human amniotic membrane. J Biol Chem. 2014;289(19):13531‑13542.
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• Cooke M, Tan EK, Mandrycky C, He H, O’Connell J, Tseng SC. Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated aminotic membrane/chorion tissue. Journal of Wound Care. 2014;23(10):465-476.